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mouse anti pdf antibody  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti pdf antibody
    Mouse Anti Pdf Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 92/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+pdf+antibody/anti-Pigment-dispersing+factor+neuropeptide/pm41701772-304-8-13
    Average 92 stars, based on 96 article reviews
    mouse anti pdf antibody - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    other:

    Article Title: Circadian modulation of mosquito host-seeking persistence by Pigment-Dispersing Factor impacts daily biting patterns
    Article Snippet: The following antibodies were used: mouse anti-PDF antibody (DSHB) at 1:1000 dilution (1:10,000 dilution in ); rat anti-mouse CD8 (Invitrogen) at 1:100 dilution, rabbit anti-GFP (Invitrogen) at 1:1000 dilution; guinea pig anti-ILP3 (this study) at 1:500 dilution.

    Staining:

    Article Title: Octopamine signaling from clock neurons plays dual roles in Drosophila long-term memory
    Article Snippet: For Tdc2 staining, brains were stained with a rabbit anti-Tdc2 antibody (ab128225, Abcam, 1/250 or pAB0822-P, Covalab, 1/250) followed by Alexa Fluor Plus 555 anti-rabbit IgG (A32732, Thermo Fisher Scientific, USA) as the secondary antibody (1:1000). .. For Pdf staining, brains were stained with a mouse anti-Pdf antibody (PDF C7-s, Developmental Studies Hybridoma Bank at the University of Iowa, 1:200) followed by Alexa Fluor 568 anti-mouse IgG (A11004, Thermo Fisher Scientific) as the secondary antibody (1:1000). .. For GFP staining, brains were stained with a chicken anti-GFP antibody (ab13970, Abcam, USA; 1:1000), followed by Alexa Fluor 488 anti-chicken IgG (A11039, Thermo Fisher Scientific, USA; 1:1000) as the secondary antibody.

    Article Title: Octopamine signaling from clock neurons plays dual roles in Drosophila long-term memory
    Article Snippet: For Tdc2 staining, brains were stained with a rabbit anti-Tdc2 antibody (ab128225, Abcam, 1/250 or pAB0822-P, Covalab, 1/250) followed by Alexa Fluor Plus 555 anti-rabbit IgG (A32732, Thermo Fisher Scientific, USA) as the secondary antibody (1:1000). .. For Pdf staining, brains were stained with a mouse anti-Pdf antibody (PDF C7-s, Developmental Studies Hybridoma Bank at the University of Iowa, 1:200) followed by Alexa Fluor 568 anti-mouse IgG (A11004, Thermo Fisher Scientific) as the secondary antibody (1:1000). .. For GFP staining, brains were stained with a chicken anti-GFP antibody (ab13970, Abcam, USA; 1:1000), followed by Alexa Fluor 488 anti-chicken IgG (A11039, Thermo Fisher Scientific, USA; 1:1000) as the secondary antibody.

    Article Title: Octopamine signaling from clock neurons plays dual roles in Drosophila long-term memory.
    Article Snippet: For Tdc2 staining, brains were stained with a rabbit anti-Tdc2 antibody (ab128225, Abcam, 1/250 or pAB0822-P, Covalab, 1/250) followed by Alexa Fluor Plus 555 anti-rabbit IgG (A32732, Thermo Fisher Scientific, USA) as the secondary antibody (1:1000). .. For Pdf staining, brains were stained with a mouse anti-Pdf antibody (PDF C7-s, Developmental Studies Hybridoma Bank at the University of Iowa, 1:200) followed by Alexa Fluor 568 anti-mouse IgG (A11004, Thermo Fisher Scientific) as the secondary antibody (1:1000). .. For GFP staining, brains were stained with a chicken anti-GFP antibody (ab13970, Abcam, USA; 1:1000), followed by Alexa Fluor 488 anti-chicken IgG (A11039, Thermo Fisher Scientific, USA; 1:1000) as the secondary antibody.

    Incubation:

    Article Title: Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae.
    Article Snippet: .. After backfills, the CNS was fixed with 4% PFA overnight, rinsed 3 times with 0.1 M PBST, and incubated in mouse anti-PDF antibody (1:100, RRID:AB_760350, PDF C7, DSHB) for 3 d at 4 °C. .. The CNS was then incubated with Alexa Fluor 488 labeled goat anti-mouse IgG (1:200, A11001, Thermo Fisher Scientific) and an avidin–biotin complex (PK-4000, Vector Lab.) at 4 °C for 1 d followed by streptavidin Alexa fluor 647 (1: 200, S21374, Thermo Fisher Scientific) for overnight at 4 °C, and then processed for dehydrated with an ethanol series and cleared with methyl salicylate.

    Article Title: Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae
    Article Snippet: .. After backfills, the CNS was fixed with 4% PFA overnight, rinsed 3 times with 0.1 M PBST, and incubated in mouse anti-PDF antibody (1:100, RRID:AB_760350, PDF C7, DSHB) for 3 d at 4 °C. .. The CNS was then incubated with Alexa Fluor 488 labeled goat anti-mouse IgG (1:200, A11001, Thermo Fisher Scientific) and an avidin–biotin complex (PK-4000, Vector Lab.) at 4 °C for 1 d followed by streptavidin Alexa fluor 647 (1: 200, S21374 , Thermo Fisher Scientific) for overnight at 4 °C, and then processed for dehydrated with an ethanol series and cleared with methyl salicylate.

    Article Title: Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae
    Article Snippet: .. The CNS was blocked in 5% NDS for 1 h at RT, and incubated in mouse anti-PDF antibody (1:100, RRID:AB_760350, PDF C7, DSHB, Iowa, USA) for 3 d at 4 °C. ..

    Article Title: Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae.
    Article Snippet: .. The CNS was blocked in 5% NDS for 1 h at RT, and incubated in mouse anti-PDF antibody (1:100, RRID:AB_760350, PDF C7, DSHB, Iowa, USA) for 3 d at 4 °C. ..



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